Temperature plays very important role in enzyme production
Temperature plays very important role in enzyme production. The optimal temperature for laccase production varies among different strains. For laccase production optimum temperature generally varies from 25°C to 30°C 50. Laccase enzyme from T. modesta was found to be fully active at 40°C and stable at 50°C but at higher temperature (60°C) half life decreased to 120 min as reported by Nyanhongo et al. 53
Influence of Ph
The pH effect is limited in case of laccase production 19.The pH values varies among the substrates used because different substrates cause different reaction for laccase. Laccase production was found to be high when fungi are grown in the medium of Ph 5.0 but some studies also reported that laccase production was found to be more suitable when enzyme production operated at pH between 4.5 to 6.0 19.
Influence of agitator
Laccase production is affected by agitation. Hess et al. 58 found that due to agitation fungal mycelia get damaged as a result of which laccase production from Trametes multicolor decreased considerably. Tavares et al. 60 found that agitation does not play any role in laccase production from T. versicolor.
Influence of inducers
Laccase production has been seen to be highly dependent on fungus cultivation 61. Laccases are generally produced in low concentrations by laccase producing fungi 39 but higher concentrations were obtained by addition of certain inducers 63,64 A new basidiomycete, Trametes sp. 420, produced laccase in glucose medium and in cellobiose medium with induction by 0.5mMand 6mMo-toluidine 70.
The promoter region encoding for laccase contains various recognition sites that are specific for xenobiotic and heavy metals 66; they bind to the recognition sites and induce laccase production.